![](/images/graphics-bg.png)
Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium
Joint Authors
Siegel, Julian
Walker, Tobias
Hartmann, Silke
Steger, Volker
Kornberger, Angela
Nolte, Andrea
Wendel, Hans-Peter
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Adhesion molecules are known to influence postoperative organ function, they are hardly involved in the inflammatory response following the ischemia-reperfusion injury.
We sought to investigate the potency of small interfering RNAs to suppress adhesion molecule expression in human pulmonary microvascular endothelial cells.
Methods.
Human lung microvascular endothelial cells were transfected with specific siRNA followed by a stimulation of the cells with an inflammatory cytokine.
Adhesion molecule expression was determined by FACS-analysis, and reduction of intracellular mRNA was determined by qRT-PCR.
Furthermore, the attachment of isolated neutrophils on the endothelial layer was determined after siRNA transfection.
Results.
In summary, siRNA transfection significantly decreased the percentage positive cells in a single cocktail transfection of each adhesion molecule investigated.
Adhering neutrophils were diminished as well.
Conclusion.
siRNA might be a promising tool for the effective suppression of adhesion molecule expression on pulmonary microvascular cells, potentially minimizing leukocyte-endothelial depending interactions of a pulmonary allograft.
American Psychological Association (APA)
Walker, Tobias& Siegel, Julian& Nolte, Andrea& Hartmann, Silke& Kornberger, Angela& Steger, Volker…[et al.]. 2011. Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-491210
Modern Language Association (MLA)
Walker, Tobias…[et al.]. Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium. Journal of Nucleic Acids No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-491210
American Medical Association (AMA)
Walker, Tobias& Siegel, Julian& Nolte, Andrea& Hartmann, Silke& Kornberger, Angela& Steger, Volker…[et al.]. Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-491210
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491210