RNA-Based Assessment of Diversity and Composition of Active Archaeal Communities in the German Bight
Joint Authors
Daniel, Rolf
Wemheuer, Franziska
Wemheuer, Bernd
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Archaea play an important role in various biogeochemical cycles.
They are known extremophiles inhabiting environments such as thermal springs or hydrothermal vents.
Recent studies have revealed a significant abundance of Archaea in moderate environments, for example, temperate sea water.
Nevertheless, the composition and ecosystem function of these marine archaeal communities is largely unknown.
To assess diversity and composition of active archaeal communities in the German Bight, seven marine water samples were taken and studied by RNA-based analysis of ribosomal 16S rRNA.
For this purpose, total RNA was extracted from the samples and converted to cDNA.
Archaeal community structures were investigated by pyrosequencing-based analysis of 16S rRNA amplicons generated from cDNA.
To our knowledge, this is the first study combining next-generation sequencing and metatranscriptomics to study archaeal communities in marine habitats.
The pyrosequencing-derived dataset comprised 62,045 archaeal 16S rRNA sequences.
We identified Halobacteria as the predominant archaeal group across all samples with increased abundance in algal blooms.
Thermoplasmatales (Euryarchaeota) and the Marine Group I (Thaumarchaeota) were identified in minor abundances.
It is indicated that archaeal community patterns were influenced by environmental conditions.
American Psychological Association (APA)
Wemheuer, Bernd& Wemheuer, Franziska& Daniel, Rolf. 2012. RNA-Based Assessment of Diversity and Composition of Active Archaeal Communities in the German Bight. Archaea،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-491301
Modern Language Association (MLA)
Wemheuer, Bernd…[et al.]. RNA-Based Assessment of Diversity and Composition of Active Archaeal Communities in the German Bight. Archaea No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-491301
American Medical Association (AMA)
Wemheuer, Bernd& Wemheuer, Franziska& Daniel, Rolf. RNA-Based Assessment of Diversity and Composition of Active Archaeal Communities in the German Bight. Archaea. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-491301
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491301