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Expression of Hepatitis B Virus Surface Antigen Containing Y100C Variant Frequently Detected in Occult HBV Infection
Joint Authors
Martel, Nora
Mello, Francisco C. A.
Gomes, Selma A.
Araujo, Natalia M.
Source
Hepatitis Research and Treatment
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-06
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Small hepatitis B virus surface protein (S-HBsAg) variant Y100C has been associated with HBsAg-negative phenotype.
To determine whether Y100C substitution yields impaired HBsAg or small amounts of HBsAg that may reduce HBsAg detection by commercial anti-HBsAg antibodies, two eukaryotic expression plasmids, one containing a wild-type S and the other an S gene from a Y100C variant, were constructed and their levels of HBsAg compared by ELISA after transfection of HuH7 cells.
Unexpectedly, the extracellular HBsAg levels detected with Y100C plasmid were higher than those observed with the wild-type plasmid, but without statistical significance.
We concluded that the Y100C substitution alone did not play a role in reducing HBsAg amounts or HBsAg affinity by commercial ELISA assay.
Further studies on in vitro replication fitness with the complete genome of HBV isolates displaying or not Y100C substitution may elucidate whether this mutation affects HBV replication and consequently HBsAg production.
American Psychological Association (APA)
Mello, Francisco C. A.& Martel, Nora& Gomes, Selma A.& Araujo, Natalia M.. 2011. Expression of Hepatitis B Virus Surface Antigen Containing Y100C Variant Frequently Detected in Occult HBV Infection. Hepatitis Research and Treatment،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-491309
Modern Language Association (MLA)
Mello, Francisco C. A.…[et al.]. Expression of Hepatitis B Virus Surface Antigen Containing Y100C Variant Frequently Detected in Occult HBV Infection. Hepatitis Research and Treatment No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-491309
American Medical Association (AMA)
Mello, Francisco C. A.& Martel, Nora& Gomes, Selma A.& Araujo, Natalia M.. Expression of Hepatitis B Virus Surface Antigen Containing Y100C Variant Frequently Detected in Occult HBV Infection. Hepatitis Research and Treatment. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-491309
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491309