Reduction of Adherence of E. coli O157 : H7 to HEp-2 Cells and to Bovine Large Intestinal Mucosal Explants by Colicinogenic E. coli

Abstract EN

Enterohemorrhagic E.

coli strains (EHEC) had emerged as foodborne pathogens and cause in human diarrhea and hemolytic-uremic syndrome.

Because of the widespread distribution of EHEC serotypes and O157 and non-O157 in cattle population, its control will require interventions at the farm level such as the administration of probiotics that produce inhibitory metabolites.

E.

coli O157:H7 shows tissue tropisms for the gastrointestinal tract (GIT) of cattle.

The aim of this study was to test the ability of a colicinogenic E.

coli (isolated from bovine) to reduce the adherence of E.

coli O157:H7 to HEp-2 cells and to GIT of cattle.

We inoculated HEp-2 cells and bovine colon explants with both kinds of strains.

Colicinogenic E.

coli was able to reduce the adherence of E.

coli O157:H7 to HEp-2 cells and to bovine tissues.