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Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana
Joint Authors
Hitomi, Kenichi
Iwai, Shigenori
Fuss, Jill O.
Fujiwara, Yoshie
Kashiwagi, Sayo
Kuraoka, Isao
Masutani, Chikahide
Shin, David S.
Cheng, Quen J.
Hanaoka, Fumio
Tainer, John A.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Human DNA polymerase η (HsPolη) plays an important role in translesion synthesis (TLS), which allows for replication past DNA damage such as UV-induced cis-syn cyclobutane pyrimidine dimers (CPDs).
Here, we characterized ApPolη from the thermophilic worm Alvinella pompejana, which inhabits deep-sea hydrothermal vent chimneys.
ApPolη shares sequence homology with HsPolη and contains domains for binding ubiquitin and proliferating cell nuclear antigen.
Sun-induced UV does not penetrate Alvinella's environment; however, this novel DNA polymerase catalyzed efficient and accurate TLS past CPD, as well as 7,8-dihydro-8-oxoguanine and isomers of thymine glycol induced by reactive oxygen species.
In addition, we found that ApPolη is more thermostable than HsPolη, as expected from its habitat temperature.
Moreover, the activity of this enzyme was retained in the presence of a higher concentration of organic solvents.
Therefore, ApPolη provides a robust, human-like Polη that is more active after exposure to high temperatures and organic solvents.
American Psychological Association (APA)
Kashiwagi, Sayo& Kuraoka, Isao& Fujiwara, Yoshie& Hitomi, Kenichi& Cheng, Quen J.& Fuss, Jill O.…[et al.]. 2010. Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-491626
Modern Language Association (MLA)
Kashiwagi, Sayo…[et al.]. Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana. Journal of Nucleic Acids No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-491626
American Medical Association (AMA)
Kashiwagi, Sayo& Kuraoka, Isao& Fujiwara, Yoshie& Hitomi, Kenichi& Cheng, Quen J.& Fuss, Jill O.…[et al.]. Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-491626
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491626