![](/images/graphics-bg.png)
Combined RASSF1A and RASSF2A Promoter Methylation Analysis as Diagnostic Biomarker for Bladder Cancer
Joint Authors
Chakravarti, Arnab
Lautenschlaeger, Tim
Huebner, Alexander
Meng, Wei
Shabsigh, Ahmad
Source
Molecular Biology International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Promoter hypermethylation, a widely studied epigenetic event known to influence gene expression levels, has been proposed as a potential biomarker in multiple types of cancer.
Clinical diagnostic biomarkers are needed for reliable prediction of bladder cancer recurrence.
In this paper, DNA promoter methylation of five C-terminal Ras-association family members (RASSF1A, RASSF2A, RASSF4, RASSF5, and RASSF6) was studied in 64 formalin-fixed paraffin-embedded (FFPE) bladder cancer and normal adjacent tissues using methylation-specific high-resolution melting (MS-HRM) analysis.
Results showed that 73% (30/41) of transitional cell carcinoma, 100% (3/3) of squamous cell carcinoma, and 100% (4/4) of small cell carcinoma demonstrated promoter methylation of the RASSF1A or RASSF2A gene, but only 6% (1/16) of normal tissues had promoter methylation of RASSF genes.
Testing positive for hypermethylation of RASSF1A or RASSF2A promoter provided 77% sensitivity and 94% specificity for identification of cancer tissues with an area under the curve of 0.854, suggesting that promoter methylation analysis of RASSF1A and RASSF2A genes has potential for use as a recurrence biomarker for bladder cancer patients.
American Psychological Association (APA)
Meng, Wei& Huebner, Alexander& Shabsigh, Ahmad& Chakravarti, Arnab& Lautenschlaeger, Tim. 2012. Combined RASSF1A and RASSF2A Promoter Methylation Analysis as Diagnostic Biomarker for Bladder Cancer. Molecular Biology International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-491666
Modern Language Association (MLA)
Meng, Wei…[et al.]. Combined RASSF1A and RASSF2A Promoter Methylation Analysis as Diagnostic Biomarker for Bladder Cancer. Molecular Biology International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-491666
American Medical Association (AMA)
Meng, Wei& Huebner, Alexander& Shabsigh, Ahmad& Chakravarti, Arnab& Lautenschlaeger, Tim. Combined RASSF1A and RASSF2A Promoter Methylation Analysis as Diagnostic Biomarker for Bladder Cancer. Molecular Biology International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-491666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491666