Evaluation of Three Formulations of Culture Media for Isolation of Brucella spp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms

Joint Authors

Lara, Gustavo H. B.
Antunes, João M. A. P.
Allendorf, Susan D.
Listoni, Fernando J. P.
Megid, Jane
Vicente, Acácia F.
Appolinário, Camila M.
Ribeiro, Marcio G.
Mioni, Mateus S. R.
Peres, Marina G.

Source

BioMed Research International

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-3, 3 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-04-10

Country of Publication

Egypt

No. of Pages

3

Main Subjects

Medicine

Abstract EN

Three culture media (Brucella agar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination and for isolating Brucella spp.

One hundred lymph nodes from pigs (n = 50) and wild boars (n = 50) with lymphadenitis were collected in slaughterhouses in the State of São Paulo and were assessed on these three selective media for Brucella spp.

All of the samples were negative for Brucella spp.

on the three culture media.

On the agar medium, fungal (70 plates) and Gram-positive bacterial (59 plates) contaminants were observed; in the CITA medium, the absence of fungal and Gram-positive bacteria on 15 plates was observed; no bacterial or fungal growth was observed on the Farrell media.

The results demonstrated that the CITA and Farrell media inhibited the growth of contaminants better than the Brucella agar.

American Psychological Association (APA)

Vicente, Acácia F.& Antunes, João M. A. P.& Lara, Gustavo H. B.& Mioni, Mateus S. R.& Allendorf, Susan D.& Peres, Marina G.…[et al.]. 2014. Evaluation of Three Formulations of Culture Media for Isolation of Brucella spp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms. BioMed Research International،Vol. 2014, no. 2014, pp.1-3.
https://search.emarefa.net/detail/BIM-491695

Modern Language Association (MLA)

Vicente, Acácia F.…[et al.]. Evaluation of Three Formulations of Culture Media for Isolation of Brucella spp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms. BioMed Research International No. 2014 (2014), pp.1-3.
https://search.emarefa.net/detail/BIM-491695

American Medical Association (AMA)

Vicente, Acácia F.& Antunes, João M. A. P.& Lara, Gustavo H. B.& Mioni, Mateus S. R.& Allendorf, Susan D.& Peres, Marina G.…[et al.]. Evaluation of Three Formulations of Culture Media for Isolation of Brucella spp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-3.
https://search.emarefa.net/detail/BIM-491695

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-491695