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Garlic Increases Antioxidant Levels in Diabetic and Hypertensive Rats Determined by a Modified Peroxidase Method
Joint Authors
Ali, Muslim
Drobiova, Hana
Peltonen-Shalaby, Riitta
Thomson, Martha
al-Qattan, Khaled
al-Amin, Zainab
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Oxidative damage by free radicals has been implicated in the pathogenesis of vascular disease in diabetes and hypertension.
In the present study, the total antioxidant status in diabetic and hypertensive rats before and after treatment with garlic (Allium sativum) was determined.
The total serum antioxidants were measured by a modified method reported earlier by Miller and coworkers.
The reproducibility of the assay was confirmed by determining standard curves for the known antioxidants: trolox (a stable analog of vitamin E), glutathione and vitamin C with interassay correlation coefficients (R2, n = 10 in triplicate) of 0.9984, 0.9768 and 0.987, respectively, confirming the reliability and reproducibility of the assay.
This assay was then used to determine total serum antioxidant levels of streptozotocin-induced diabetic rats and two-kidney one-clip hypertensive rats both before and after 3 weeks of treatment with an aqueous extract of garlic (500 mg/kg IP daily).
The serum antioxidant levels of rats after 3 weeks of treatment were significantly higher (P < .001) than the pretreatment levels in both diabetic and hypertensive rats.
The increased serum antioxidant levels were paralleled by a decrease in serum glucose in the garlic-treated diabetic rats and lowered systolic blood pressure in the garlic-treated hypertensive rats.
We conclude from our study that (i) total antioxidants can be measured by a simple, reproducible, reliable assay and (ii) the total antioxidant status can be significantly improved by treatment with garlic.
American Psychological Association (APA)
Drobiova, Hana& Thomson, Martha& al-Qattan, Khaled& Peltonen-Shalaby, Riitta& al-Amin, Zainab& Ali, Muslim. 2010. Garlic Increases Antioxidant Levels in Diabetic and Hypertensive Rats Determined by a Modified Peroxidase Method. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-491789
Modern Language Association (MLA)
Drobiova, Hana…[et al.]. Garlic Increases Antioxidant Levels in Diabetic and Hypertensive Rats Determined by a Modified Peroxidase Method. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-491789
American Medical Association (AMA)
Drobiova, Hana& Thomson, Martha& al-Qattan, Khaled& Peltonen-Shalaby, Riitta& al-Amin, Zainab& Ali, Muslim. Garlic Increases Antioxidant Levels in Diabetic and Hypertensive Rats Determined by a Modified Peroxidase Method. Evidence-Based Complementary and Alternative Medicine. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-491789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491789