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Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis
Joint Authors
de Albuquerque de Carvalho, Marcelle Lins
Melo Santa Anna, Lidia Maria
de Castro, Aline Machado
Pereira, Nei
Carvalho, Daniele Fernandes
de Barros Gomes, Edelvio
Nobuyuki Maeda, Roberto
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Increasing interest in the production of second-generation ethanol necessitates the low-cost production of enzymes from the cellulolytic complex (endoglucanases, exoglucanases, and β-glucosidases), which act synergistically in cellulose breakdown.
The present work aimed to optimise a bioprocess to produce these biocatalysts from the fungus Penicillium funiculosum ATCC11797.
A statistical full factorial design (FFD) was employed to determine the optimal conditions for cellulase production.
The optimal composition of culture media using Avicel (10 g·L−1) as carbon source was determined to include urea (1.2 g·L−1), yeast extract (1.0 g·L−1), KH2PO4 (6.0 g·L−1), and MgSO4·7H2O (1.2 g·L−1).
The growth process was performed in batches in a bioreactor.
Using a different FFD strategy, the optimised bioreactor operational conditions of an agitation speed of 220 rpm and aeration rate of 0.6 vvm allowed the obtainment of an enzyme pool with activities of 508 U·L−1 for FPase, 9,204 U·L−1 for endoglucanase, and 2,395 U·L−1 for β-glucosidase.
The sequential optimisation strategy was effective and afforded increased cellulase production in the order from 3.6 to 9.5 times higher than production using nonoptimised conditions.
American Psychological Association (APA)
de Albuquerque de Carvalho, Marcelle Lins& Carvalho, Daniele Fernandes& de Barros Gomes, Edelvio& Nobuyuki Maeda, Roberto& Melo Santa Anna, Lidia Maria& de Castro, Aline Machado…[et al.]. 2014. Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis. Enzyme Research،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-491815
Modern Language Association (MLA)
de Albuquerque de Carvalho, Marcelle Lins…[et al.]. Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis. Enzyme Research No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-491815
American Medical Association (AMA)
de Albuquerque de Carvalho, Marcelle Lins& Carvalho, Daniele Fernandes& de Barros Gomes, Edelvio& Nobuyuki Maeda, Roberto& Melo Santa Anna, Lidia Maria& de Castro, Aline Machado…[et al.]. Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis. Enzyme Research. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-491815
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491815