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Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells
Joint Authors
Dunne, Margaret R.
Doherty, Derek G.
Mangan, Bozgana A.
Madrigal-Estebas, Laura
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Human Vγ9Vδ2 T cells recognise pyrophosphate-based antigens (phosphoantigens) and have multiple functions in innate and adaptive immunity, including a unique ability to activate other cells of the immune system.
We used flow cytometry and ELISA to define the early cytokine profiles of Vγ9Vδ2 T cells stimulated in vitro with isopentenyl pyrophosphate (IPP) and (E)-4-hydroxy-3-methyl-but-2 enyl pyrophosphate (HMB-PP) in the absence and presence of IL-2 and IL-15.
We show that fresh Vγ9Vδ2 T cells produce interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α) within 4 hours of stimulation with phosphoantigen, but neither IL-10, IL-13, nor IL-17 was detectable up to 72 hours under these conditions.
Cytokine production was not influenced by expression or lack, thereof, of CD4 or CD8.
Addition of IL-2 or IL-15 caused expansion of IFN-γ-producing Vγ9Vδ2 T cells, but did not enhance IFN-γ secretion after 24–72 hours.
Thus, phosphoantigen-stimulated Vγ9Vδ2 T cells have potential as Th1-biasing adjuvants for immunotherapy.
American Psychological Association (APA)
Dunne, Margaret R.& Mangan, Bozgana A.& Madrigal-Estebas, Laura& Doherty, Derek G.. 2011. Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells. Mediators of Inflammation،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-491961
Modern Language Association (MLA)
Dunne, Margaret R.…[et al.]. Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells. Mediators of Inflammation No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-491961
American Medical Association (AMA)
Dunne, Margaret R.& Mangan, Bozgana A.& Madrigal-Estebas, Laura& Doherty, Derek G.. Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells. Mediators of Inflammation. 2011. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-491961
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491961