Characterization of Modified Magnetite Nanoparticles for Albumin Immobilization
Joint Authors
Amiri, R.
Ranjbakhsh, E.
Bordbar, A. K.
Abbasi, M.
Khosropour, A. R.
Rastegari, A. A.
Source
Biotechnology Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Information Technology and Computer Science
Abstract EN
Magnetite Fe3O4 nanoparticles (NPs) were prepared by chemical coprecipitation method.
Silica-coated magnetite NPs were prepared by sol-gel reaction, subsequently coated with 3-aminopropyltriethoxysilane (APTES) via silanization reaction, and then were activated with 2,4,6-trichloro-1,3,5-triazine (TCT) and covalently immobilized with bovine serum albumin (BSA).
The size and structure of the particles were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and dynamic light scattering (DLS) techniques.
The immobilization was confirmed by Fourier transform infrared spectroscopy (FT-IR).
XRD analysis showed that the binding process has not done any phase change to Fe3O4.
The immobilization time for this process was 4 h and the amount of immobilized BSA for the initial value of 1.05 mg BSA was about 120 mg/gr nanoparticles.
Also, the influences of three different buffer solutions and ionic strength on covalent immobilization were evaluated.
American Psychological Association (APA)
Bordbar, A. K.& Rastegari, A. A.& Amiri, R.& Ranjbakhsh, E.& Abbasi, M.& Khosropour, A. R.. 2014. Characterization of Modified Magnetite Nanoparticles for Albumin Immobilization. Biotechnology Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-491976
Modern Language Association (MLA)
Bordbar, A. K.…[et al.]. Characterization of Modified Magnetite Nanoparticles for Albumin Immobilization. Biotechnology Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-491976
American Medical Association (AMA)
Bordbar, A. K.& Rastegari, A. A.& Amiri, R.& Ranjbakhsh, E.& Abbasi, M.& Khosropour, A. R.. Characterization of Modified Magnetite Nanoparticles for Albumin Immobilization. Biotechnology Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-491976
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491976
