The PPARδ Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells
Joint Authors
Nasrallah, Rania
Clark, Jordan
Hébert, Richard L.
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-03-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The collecting duct (CD) expresses considerable amounts of PPARδ.
While its role is unknown in the CD, in other renal cells it has been shown to regulate both growth and apoptosis.
We thus hypothesized that PPARδ reduces apoptotic responses and stimulates cell growth in the mouse CD, and examined the effect of GW501516, a synthetic PPARδ ligand, on these responses in mouse IMCD-K2 cells.
High doses of GW501516 decreased both DNA and protein synthesis in these cells by 80%, but had no overall effect on cell viability.
Although anisomycin treatment resulted in an increase of caspase-3 levels of about 2.59-fold of control, GW501516 did not affect anisomycin-induced changes in active caspase-3 levels.
These results show that a PPARδ ligand inhibits growth but does not affect anisomycin-apoptosis in a mouse IMCD cell line.
This could have therapeutic implications for renal diseases associated with increased CD growth responses.
American Psychological Association (APA)
Clark, Jordan& Nasrallah, Rania& Hébert, Richard L.. 2009. The PPARδ Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells. PPAR Research،Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-492087
Modern Language Association (MLA)
Clark, Jordan…[et al.]. The PPARδ Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells. PPAR Research No. 2009 (2009), pp.1-11.
https://search.emarefa.net/detail/BIM-492087
American Medical Association (AMA)
Clark, Jordan& Nasrallah, Rania& Hébert, Richard L.. The PPARδ Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells. PPAR Research. 2009. Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-492087
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492087