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Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification
Joint Authors
Glennon, Erin
Ching, Wei-Mei
Chen, Hua-Wei
Zhang, Zhiwen
Source
International Journal of Bacteriology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Currently, the accepted method for Q fever serodiagnosis is indirect immunofluorescent antibody assay (IFA) using the whole cell antigen.
In this study, we prepared the recombinant antigen of the 27-kDa outer membrane protein (Com1) which has been shown to be recognized by Q fever patient sera.
The performance of recombinant Com1 was evaluated in ELISA by IFA confirmed serum samples.
Due to the low titers of IgG and IgM in Q fever patients, the standard ELISA signals were further amplified by using biotinylated anti-human IgG or IgM plus streptavidin-HRP polymer.
The modified ELISA can detect 88% (29 out of 33) of Q fever patient sera collected from Marines deployed to Iraq.
Less than 5% (5 out of 156) of the sera from patients with other febrile diseases reacted with the Com1.
These results suggest that the modified ELISA using Com1 may have the potential to improve the detection of Q fever specific antibodies.
American Psychological Association (APA)
Chen, Hua-Wei& Zhang, Zhiwen& Glennon, Erin& Ching, Wei-Mei. 2014. Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification. International Journal of Bacteriology،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-492178
Modern Language Association (MLA)
Chen, Hua-Wei…[et al.]. Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification. International Journal of Bacteriology No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-492178
American Medical Association (AMA)
Chen, Hua-Wei& Zhang, Zhiwen& Glennon, Erin& Ching, Wei-Mei. Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification. International Journal of Bacteriology. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-492178
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492178