Withaferin A Induces Proteasome-Dependent Degradation of Breast Cancer Susceptibility Gene 1 and Heat Shock Factor 1 Proteins in Breast Cancer Cells
Joint Authors
Timmermann, Barbara
Cohen, Mark S.
Samadi, Abbas K.
Zhang, Xuan
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The purpose of this study was to examine the regulation of prosurvival factors heat shock factor 1 (HSF1) and breast cancer susceptibility gene 1 (BRCA1) by a natural withanolide withaferin A (WA) in triple negative breast cancer cell lines MDA-MB-231 and BT20.
Western analysis was used to examine alternations in HSF1 and BRCA1 protein levels following WA treatment.
A protein synthesis inhibitor cycloheximide and a proteasome inhibitor MG132 were used to investigate the mechanisms of HSF1 and BRCA1 regulation by WA.
It was found that WA induced a dose-dependent decrease in HSF1 and BRCA1 protein levels.
Further analysis showed that levels of HSF1 and BRCA1 proteins decreased rapidly after WA treatment, and this was attributed to WA-induced denaturation of HSF1 and BRCA1 proteins and subsequent degradation via proteasome-dependent, and protein-synthesis dependent mechanism.
In summary, WA induces denaturation and proteasomal degradation of HSF1 and BRCA1 proteins.
Further studies are warranted to examine the contribution of HSF1 and BRCA1 depletion to the anticancer effects of WA in breast cancer.
American Psychological Association (APA)
Zhang, Xuan& Timmermann, Barbara& Samadi, Abbas K.& Cohen, Mark S.. 2012. Withaferin A Induces Proteasome-Dependent Degradation of Breast Cancer Susceptibility Gene 1 and Heat Shock Factor 1 Proteins in Breast Cancer Cells. ISRN Biochemistry،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-492191
Modern Language Association (MLA)
Zhang, Xuan…[et al.]. Withaferin A Induces Proteasome-Dependent Degradation of Breast Cancer Susceptibility Gene 1 and Heat Shock Factor 1 Proteins in Breast Cancer Cells. ISRN Biochemistry No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-492191
American Medical Association (AMA)
Zhang, Xuan& Timmermann, Barbara& Samadi, Abbas K.& Cohen, Mark S.. Withaferin A Induces Proteasome-Dependent Degradation of Breast Cancer Susceptibility Gene 1 and Heat Shock Factor 1 Proteins in Breast Cancer Cells. ISRN Biochemistry. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-492191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492191