Uptake of Etoposide in CT-26 Cells of Colorectal Cancer Using Folate Targeted Dextran Stearate Polymeric Micelles
Joint Authors
Varshosaz, Jaleh
Firozian, Farzin
Hassanzadeh, Farshid
Sadeghi-Aliabadi, Hojjat
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Targeted drug delivery using folate receptors is one of the most interesting chemotherapeutic research areas over the past few years.
A novel folate targeted copolymer was synthesized using dextran stearate coupled to folic acid.
FT-IR and NMR spectroscopy were used to confirm successful conjugation.
Micelles prepared using this copolymer were characterized for their particle size, zeta potential, critical micelle concentration (CMC), drug loading capacity, and release efficiency.
Cytotoxicity and cellular uptake of the micelles were estimated using CT-26 colorectal carcinoma cell line.
FT-IR and NMR spectroscopy confirmed production of folate grafted dextran stearate copolymer.
Low CMC value indicates that the copolymers are suitable for preparation of stable micelles useful in parenteral dosage forms.
Particle size and zeta potential of the targeted nanoparticles were 105.5±2.0 nm and −21.2 mV, respectively.
IC50 of etoposide loaded in folate grafted dextran stearate enhanced about 20-fold compared to the pure drug (0.49±0.11 μg/mL versus 9.41±0.52 μg/mL).
It seems that etoposide loaded in micelles of folate grafted dextran stearate copolymer is promising in reducing drug resistance of colorectal cancer by boosting etoposide cellular uptake.
American Psychological Association (APA)
Varshosaz, Jaleh& Hassanzadeh, Farshid& Sadeghi-Aliabadi, Hojjat& Firozian, Farzin. 2014. Uptake of Etoposide in CT-26 Cells of Colorectal Cancer Using Folate Targeted Dextran Stearate Polymeric Micelles. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-492276
Modern Language Association (MLA)
Varshosaz, Jaleh…[et al.]. Uptake of Etoposide in CT-26 Cells of Colorectal Cancer Using Folate Targeted Dextran Stearate Polymeric Micelles. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-492276
American Medical Association (AMA)
Varshosaz, Jaleh& Hassanzadeh, Farshid& Sadeghi-Aliabadi, Hojjat& Firozian, Farzin. Uptake of Etoposide in CT-26 Cells of Colorectal Cancer Using Folate Targeted Dextran Stearate Polymeric Micelles. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-492276
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492276