Effect of Growth Regulators on In Vitro Morphogenic Response of Boscia senegalensis (Pers.)‎ Lam. Poir. Using Mature Zygotic Embryos Explants

Abstract EN

The percent study describes the in vitro responses of mature zygotic embryos of Boscia senegalensis to different concentrations (0.0–5.0 mg/L) of 6-benzyladnine (BA), Thidiazuron (TDZ), α-Naphthalene acetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) supplemented on Murashige and Skoog medium (MS).

The plant growth regulators (PGRs) were considerably affected the morphogenetic responses.

BA produced adventitious shoots through two ways: direct organogenesis and auxiliary shoot formation.

Both 2, 4-D and TDZ tend to produce callus, whereas NAA improve the development of embryos to seedlings.

Maximum number of shoots/explant (14.8 ± 0.6) was obtained on MS medium supplemented with 3.0 mg/L BA.

67.0% of excised shoots were rooted either on 1/2 MS medium augmented with or without 0.25 mg/L IBA.

The highest number of roots (1.2 ± 0.4) and root length (0.5 ± 0.2 cm) was produced on 0.25 mg/L IBA-containing medium.

Regenerated plants were successfully acclimatized and transferred to the green house with 70% survival rate.

All the plants appeared morphologically uniform with normal growth pattern.

A rapid (30 days), efficient and without subculturing protocol for in vitro regeneration of B.

senegalensis was developed.