UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit
Joint Authors
West, Brett J.
Deng, Shixin
Jensen, C. Jarakae
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Cornus mas L.
is indigenous to Europe and parts of Asia.
Although Cornus is widely considered to be an iridoid rich genera, only two iridoids have been previously found in this plant.
The lack of information on taxonomically and biologically active iridoids prompted us to develop and optimize an analytical method for characterization of additional phytochemicals in C.
mas fruit.
An ultra performance liquid chromatography (UPLC) coupled with photodiode array spectrophotometry (PDA) and electrospray time-of-flight mass spectrometry (ESI-TOF-MS) was employed and mass parameters were optimized.
Identification was made by elucidating the mass spectral data and further confirmed by comparing retention times and UV spectra of target peaks with those of reference compounds.
Primary DNA damage and antigenotoxicity tests in E.
coli PQ37 were used to screen the iridoids for biological activity.
As a result, ten phytochemicals were identified, including iridoids loganic acid, loganin, sweroside, and cornuside.
Nine of these were reported for the first time from C.
mas fruit.
The iridoids did not induce SOS repair of DNA, indicating a lack of genotoxic activity in E.
coli PQ37.
However, loganin, sweroside, and cornuside did reduce the amount of DNA damage caused by 4-nitroquinoline 1-oxide, suggesting potential antigenotoxic activity.
American Psychological Association (APA)
Deng, Shixin& West, Brett J.& Jensen, C. Jarakae. 2013. UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-492492
Modern Language Association (MLA)
Deng, Shixin…[et al.]. UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-492492
American Medical Association (AMA)
Deng, Shixin& West, Brett J.& Jensen, C. Jarakae. UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-492492
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492492