Analysis of Some Biogenic Amines by Micellar Liquid Chromatography
Joint Authors
Stępnik, Katarzyna E.
Malinowska, Irena
Source
Chromatography Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Micellar liquid chromatography (MLC) with the use of high performance liquid chromatography (HPLC) was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine.
In this paper, an influence of surfactant’s concentration and pH of the micellar mobile phase on the retention of the tested substances was examined.
To determine the influence of surfactant’s concentration on the retention of the tested amines, buffered solutions (at pH 7.4) of ionic surfactant—sodium dodecyl sulfate SDS (at different concentrations) with acetonitrile as an organic modifier (0.8/0.2 v/v) were used as the micellar mobile phases.
To determine the influence of pH of the micellar mobile phase on the retention, mobile phases contained buffered solutions (at different pH values) of sodium dodecyl sulfate SDS (at 0.1 M) with acetonitrile (0.8/0.2 v/v).
The inverse of value of retention factor (1/k) versus concentration of micelles (CM) relationships were examined.
Other physicochemical parameters of solutes such as an association constant analyte—micelle (Kma)—and partition coefficient of analyte between stationary phase and water (hydrophobicity descriptor) (PswΦ) were determined by the use of Foley’s equation.
American Psychological Association (APA)
Malinowska, Irena& Stępnik, Katarzyna E.. 2012. Analysis of Some Biogenic Amines by Micellar Liquid Chromatography. Chromatography Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-492612
Modern Language Association (MLA)
Malinowska, Irena& Stępnik, Katarzyna E.. Analysis of Some Biogenic Amines by Micellar Liquid Chromatography. Chromatography Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-492612
American Medical Association (AMA)
Malinowska, Irena& Stępnik, Katarzyna E.. Analysis of Some Biogenic Amines by Micellar Liquid Chromatography. Chromatography Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-492612
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492612