Radiosensitization Effect of Nedaplatin on Nasopharyngeal Carcinoma Cells in Different Status of Epstein-Barr Virus Infection
Joint Authors
Wu, Jing
Wang, Xue
Wu, Jianfeng
Chen, Meng
Gu, Jiajia
Ye, Jinjun
Xu, Lin
He, Xia
Yin, Li
Wang, Dejun
Jiang, Xuesong
Guo, Wenjie
Zhang, Junying
Wu, Jianzhong
Zong, Dan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study aims to evaluate the radiosensitization effect of nedaplatin on nasopharyngeal carcinoma (NPC) cell lines with different Epstein-Barr virus (EBV) status.
Human NPC cell lines CNE-2 (EBV-negative) and C666 (EBV-positive) were treated with 0–100 μg/mL nedaplatin, and inhibitory effects on cell viability and IC50 were calculated by MTS assay.
We assessed changes in radiosensitivity of cells by MTS and colony formation assays, and detected the apoptosis index and changes in cell cycle by flow cytometry.
MTS assay showed that nedaplatin caused significant cytotoxicity in CNE-2 and C666 cells in a time- and dose-dependent manner.
After 24 h, nedaplatin inhibited growth of CNE-2 and C666 cells with IC50 values of 34.32 and 63.69 μg/mL, respectively.
Compared with radiation alone, nedaplatin enhanced the radiation effect on both cell lines.
Nedaplatin markedly increased apoptosis and cell cycle arrest in G2/M phase.
Nedaplatin radiosensitized human NPC cells CNE-2 and C666, with a significantly greater effect on the former.
The mechanisms of radiosensitization include induction of apoptosis and enhancement of cell cycle arrest in G2/M phase.
American Psychological Association (APA)
Yin, Li& Wu, Jing& Wu, Jianfeng& Ye, Jinjun& Jiang, Xuesong& Chen, Meng…[et al.]. 2014. Radiosensitization Effect of Nedaplatin on Nasopharyngeal Carcinoma Cells in Different Status of Epstein-Barr Virus Infection. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-492647
Modern Language Association (MLA)
Yin, Li…[et al.]. Radiosensitization Effect of Nedaplatin on Nasopharyngeal Carcinoma Cells in Different Status of Epstein-Barr Virus Infection. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-492647
American Medical Association (AMA)
Yin, Li& Wu, Jing& Wu, Jianfeng& Ye, Jinjun& Jiang, Xuesong& Chen, Meng…[et al.]. Radiosensitization Effect of Nedaplatin on Nasopharyngeal Carcinoma Cells in Different Status of Epstein-Barr Virus Infection. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-492647
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492647