mAb CZP-315.D9 : an antirecombinant cruzipain monoclonal antibody that specifically labels the reservosomes of Trypanosoma cruzi epimastigotes
Joint Authors
Batista, Cassiano Martin
Eger, Iriane
de Souza, Wanderley
Soares, Maurilio José
Medeiros, Lia Carolina Soares
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Reservosomes are large round vesicles located at the posterior end of epimastigote forms of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease.
They are the specific end organelles of the endocytosis pathway of T.
cruzi, and they play key roles in nutrient uptake and cell differentiation.
These lysosome-like organelles accumulate ingested macromolecules and contain large amounts of a major cysteine proteinase (cruzipain or GP57/51 protein).
Aim of this study was to produce a monoclonal antibody (mAb) against a recombinant T.
cruzi cruzipain (TcCruzipain) that specifically labels the reservosomes.
BALB/c mice were immunized with purified recombinant TcCruzipain to obtain the mAb.
After fusion of isolated splenocytes with myeloma cells and screening, a mAb was obtained by limiting dilution and characterized by capture ELISA.
We report here the production of a kappa-positive monoclonal IgG antibody (mAb CZP-315.D9) that recognizes recombinant TcCruzipain.
This mAb binds preferentially to a protein with a molecular weight of about 50 kDa on western blots and specifically labels reservosomes by immunofluorescence and transmission electron microscopy.
The monoclonal CZP-315.D9 constitutes a potentially powerful marker for use in studies on the function of reservosomes of T.
cruzi.
American Psychological Association (APA)
Batista, Cassiano Martin& Medeiros, Lia Carolina Soares& Eger, Iriane& Soares, Maurilio José& de Souza, Wanderley. 2014. mAb CZP-315.D9 : an antirecombinant cruzipain monoclonal antibody that specifically labels the reservosomes of Trypanosoma cruzi epimastigotes. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-492736
Modern Language Association (MLA)
Batista, Cassiano Martin…[et al.]. mAb CZP-315.D9 : an antirecombinant cruzipain monoclonal antibody that specifically labels the reservosomes of Trypanosoma cruzi epimastigotes. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-492736
American Medical Association (AMA)
Batista, Cassiano Martin& Medeiros, Lia Carolina Soares& Eger, Iriane& Soares, Maurilio José& de Souza, Wanderley. mAb CZP-315.D9 : an antirecombinant cruzipain monoclonal antibody that specifically labels the reservosomes of Trypanosoma cruzi epimastigotes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-492736
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492736