Amniotic-Fluid Stem Cells : Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure
Joint Authors
Hoopmann, M.
Arnhold, S.
Raabe, O.
Addicks, Klaus
Glüer, S.
Wenisch, S.
Hartmann, K.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Amniotic fluid (AF) has become an interesting source of fetal stem cells.
However, AF contains heterogeneous and multiple, partially differentiated cell types.
After isolation from the amniotic fluid, cells were characterized regarding their morphology and growth dynamics.
They were sorted by magnetic associated cell sorting using the surface marker CD 117.
In order to show stem cell characteristics such as pluripotency and to evaluate a possible therapeutic application of these cells, AF fluid-derived stem cells were differentiated along the adipogenic, osteogenic, and chondrogenic as well as the neuronal lineage under hypoxic conditions.
Our findings reveal that magnetic associated cell sorting (MACS) does not markedly influence growth characteristics as demonstrated by the generation doubling time.
There was, however, an effect regarding an altered adipogenic, osteogenic, and chondrogenic differentiation capacity in the selected cell fraction.
In contrast, in the unselected cell population neuronal differentiation is enhanced.
American Psychological Association (APA)
Arnhold, S.& Glüer, S.& Hartmann, K.& Raabe, O.& Addicks, Klaus& Wenisch, S.…[et al.]. 2011. Amniotic-Fluid Stem Cells : Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure. Stem Cells International،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-492795
Modern Language Association (MLA)
Arnhold, S.…[et al.]. Amniotic-Fluid Stem Cells : Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure. Stem Cells International No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-492795
American Medical Association (AMA)
Arnhold, S.& Glüer, S.& Hartmann, K.& Raabe, O.& Addicks, Klaus& Wenisch, S.…[et al.]. Amniotic-Fluid Stem Cells : Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-492795
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492795