Adrenergic Activation of Melatonin Secretion in Ovine Pineal Explants in Short-Term Superfusion Culture Occurs via Protein Synthesis Independent and Dependent Phenomena
Joint Authors
Prusik, Magdalena
Lewczuk, Bogdan
Ziółkowska, Natalia
Przybylska-Gornowicz, Barbara
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The ovine pineal is generally considered as an interesting model for the study on adrenergic regulation of melatonin secretion due to some functional similarities with this gland in the human.
The present investigations, performed in the superfusion culture of pineal explants, demonstrated that the norepinephrine-induced elevation of melatonin secretion in ovine pinealocytes comprised of two subsequent periods: a rapid increase phase and a slow increase phase.
The first one included the quick rise in release of N-acetylserotonin and melatonin, occurring parallel to elevation of NE concentration in the medium surrounding explants.
This rapid increase phase was not affected by inhibition of translation.
The second, slow increase phase began after NE level had reached the maximum concentration in the culture medium and lasted about two hours.
It was completely abolished by the treatment with translation inhibitors.
The obtained results showed for the first time that the regulation of N-acetylserotonin synthesis in pinealocytes of some species like the sheep involves the on/off mechanism, which is completely independent of protein synthesis and works very fast.
They provided strong evidence pointing to the need of revision of the current opinion that arylalkylamines N-acetyltransferase activity in pinealocytes is controlled exclusively by changes in enzyme abundance.
American Psychological Association (APA)
Lewczuk, Bogdan& Ziółkowska, Natalia& Prusik, Magdalena& Przybylska-Gornowicz, Barbara. 2014. Adrenergic Activation of Melatonin Secretion in Ovine Pineal Explants in Short-Term Superfusion Culture Occurs via Protein Synthesis Independent and Dependent Phenomena. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-492826
Modern Language Association (MLA)
Lewczuk, Bogdan…[et al.]. Adrenergic Activation of Melatonin Secretion in Ovine Pineal Explants in Short-Term Superfusion Culture Occurs via Protein Synthesis Independent and Dependent Phenomena. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-492826
American Medical Association (AMA)
Lewczuk, Bogdan& Ziółkowska, Natalia& Prusik, Magdalena& Przybylska-Gornowicz, Barbara. Adrenergic Activation of Melatonin Secretion in Ovine Pineal Explants in Short-Term Superfusion Culture Occurs via Protein Synthesis Independent and Dependent Phenomena. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-492826
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492826