Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection
Joint Authors
Ueno, Takahiro
Matsumoto, Yoshiaki
Fukuda, Noboru
Tanaka, Chihiro
Kamei, Tomonori
Nagashima, Takafumi
Aoyama, Takahiko
Hayashi, Hiroyuki
Nagase, Hiroki
Aoyama, Yukio
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A simple and robust method using high-performance liquid chromatography with UV detection was developed and validated for the determination of six pyrrole-imidazole (PI) polyamides (HN.49, TGF-β1f, TGF-β1t, HN.50f, HN.50t, and LOX-1) in rat plasma.
After the plasma proteins were precipitated with methanol containing phenacetin as an internal standard, the analytes were separated on a Luna C18 (2) (5 μm, 4.6×150 mm).
Calibration curves were linear over the range of 0.5 to 200 μg/mL for HN.49, 0.25 to 200 μg/mL for TGF-β1f, TGF-β1t, HN.50t, and LOX-1, 1 to 200 μg/mL for HN.50f in rat plasma.
The inter- and intraday precision were below 15%, and the accuracy was within 15% at the quality controls.
The validated method was successfully applied to sample analysis for the pharmacokinetic study.
American Psychological Association (APA)
Kamei, Tomonori& Aoyama, Takahiko& Tanaka, Chihiro& Nagashima, Takafumi& Aoyama, Yukio& Hayashi, Hiroyuki…[et al.]. 2012. Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-492854
Modern Language Association (MLA)
Kamei, Tomonori…[et al.]. Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-492854
American Medical Association (AMA)
Kamei, Tomonori& Aoyama, Takahiko& Tanaka, Chihiro& Nagashima, Takafumi& Aoyama, Yukio& Hayashi, Hiroyuki…[et al.]. Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-492854
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492854