Immobilization of Peroxidase onto Magnetite Modified Polyaniline
Joint Authors
Barbosa, Eduardo Fernandes
Caramori, Samantha Salomão
García-Ruíz, Pedro Antonio
Fernandes, Kátia Flávia
Lopes, Flavio Marques
Molina, Fernando Javier
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde.
After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency of 100% (active protein).
The optimum pH for immobilization was displaced by the effect of the partition of protons produced in the microenvironment by the magnetite.
The tests of repeated use have shown that PANImG-HRP can be used for 13 cycles with maintenance of 50% of the initial activity.
American Psychological Association (APA)
Barbosa, Eduardo Fernandes& Molina, Fernando Javier& Lopes, Flavio Marques& García-Ruíz, Pedro Antonio& Caramori, Samantha Salomão& Fernandes, Kátia Flávia. 2012. Immobilization of Peroxidase onto Magnetite Modified Polyaniline. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-492896
Modern Language Association (MLA)
Barbosa, Eduardo Fernandes…[et al.]. Immobilization of Peroxidase onto Magnetite Modified Polyaniline. The Scientific World Journal No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-492896
American Medical Association (AMA)
Barbosa, Eduardo Fernandes& Molina, Fernando Javier& Lopes, Flavio Marques& García-Ruíz, Pedro Antonio& Caramori, Samantha Salomão& Fernandes, Kátia Flávia. Immobilization of Peroxidase onto Magnetite Modified Polyaniline. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-492896
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492896
