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Protective Effect of Punica granatum L. against SerumGlucose Deprivation-Induced PC12 Cells Injury
Joint Authors
Sadeghnia, Hamid Reza
Ghorbani, Ahmad
Afkhami Goli, Amir
Asadpour, Elham
Forouzanfar, Fatemeh
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The discovery and development of natural products with potent antioxidant, anti-inflammatory, and antiapoptotic properties have been one of the most interesting and promising approaches in the search for the treatment of many neurodegenerative diseases including ischemic stroke.
Serum/glucose deprivation (SGD) has served as an excellent in vitro model for the understanding of the molecular mechanisms of neuronal damage during ischemia and for the development of neuroprotective drugs against ischemia-induced brain injury.
Recent studies suggested that pomegranate (Punica granatum L.) or its active constituents exert pharmacological actions such as antioxidant, anti-inflammatory, and neuroprotective properties.
Therefore, in this study we investigated the possible protective effects of different extracts of pomegranate against SGD-induced PC12 cells injury.
Initially, the cells were pretreated with different concentrations of pulp hydroalcoholic extract (PHE), pulp aqueous extract (PAE) and pomegranate juice (PJ) for 2 h and then deprived of serum/glucose (SGD) for 6 and 12 h.
SGD caused a significant reduction in cell viability (measured by the MTT assay) after 6 and 12 h, as compared with control cells (P<0.001).
Pretreatment with PHE, PAE, and PJ significantly and concentration-dependently increased cell viability following SGD insult for 6 and 12 h.
A significant increase in DNA damage (measured by the comet assay) was seen in nuclei of cells following SGD for 12 h (P<0.001).
In control groups, no significant difference was seen in DNA damage between PHE, PAE, and PJ-pretreated and vehicle-pretreated PC12 cells (P>0.05).
PHE, PAE, and PJ pretreatment resulted in a significant decrease in DNA damage following ischemic insult (P<0.001).
This suppression of DNA damage by PHE, PAE and PJ was found to be concentration dependent.
These data indicate that there is a cytoprotective property in PHE, PAE, and PJ under SGD condition in PC12 cells, suggesting that pomegranate has the potential to be used as a new therapeutic strategy for neurodegenerative disorders.
American Psychological Association (APA)
Forouzanfar, Fatemeh& Afkhami Goli, Amir& Asadpour, Elham& Ghorbani, Ahmad& Sadeghnia, Hamid Reza. 2013. Protective Effect of Punica granatum L. against SerumGlucose Deprivation-Induced PC12 Cells Injury. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-492928
Modern Language Association (MLA)
Forouzanfar, Fatemeh…[et al.]. Protective Effect of Punica granatum L. against SerumGlucose Deprivation-Induced PC12 Cells Injury. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-492928
American Medical Association (AMA)
Forouzanfar, Fatemeh& Afkhami Goli, Amir& Asadpour, Elham& Ghorbani, Ahmad& Sadeghnia, Hamid Reza. Protective Effect of Punica granatum L. against SerumGlucose Deprivation-Induced PC12 Cells Injury. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-492928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492928