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Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells
Joint Authors
Verfaillie, Catherine M.
Moriarity, Branden S.
Wilber, Andrew
Lakshmipathy, Uma
McIvor, R. Scott
Hammer, Luke
Geurts, Aron M.
Largaespada, David A.
Ulloa Montoya, Fernando
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-02
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Non-viral integrating systems, PhiC31 phage integrase (ϕC31), and Sleeping Beauty transposase (SB), provide an effective method for ex vivo gene delivery into cells.
Here, we used a plasmid-encoding GFP and neomycin phosphotransferase along with recognition sequences for both ϕC31 and SB integrating systems to demonstrate that both systems effectively mediated integration in cultured human fibroblasts and in rat multipotent adult progenitor cells (rMAPC).
Southern blot analysis of G418-resistant rMAPC clones showed a 2-fold higher number of SB-mediated insertions per clone compared to ϕC31.
Sequence identification of chromosomal junction sites indicated a random profile for SB-mediated integrants and a more restricted profile for ϕC31 integrants.
Transgenic rMAPC generated with both systems maintained their ability to differentiate into liver and endothelium albeit with marked attenuation of GFP expression.
We conclude that both SB and ϕC31 are effective non-viral integrating systems for genetic engineering of MAPC in basic studies of stem cell biology.
American Psychological Association (APA)
Wilber, Andrew& Ulloa Montoya, Fernando& Hammer, Luke& Moriarity, Branden S.& Geurts, Aron M.& Largaespada, David A.…[et al.]. 2011. Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells. Stem Cells International،Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-492964
Modern Language Association (MLA)
Wilber, Andrew…[et al.]. Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells. Stem Cells International No. 2011 (2011), pp.1-14.
https://search.emarefa.net/detail/BIM-492964
American Medical Association (AMA)
Wilber, Andrew& Ulloa Montoya, Fernando& Hammer, Luke& Moriarity, Branden S.& Geurts, Aron M.& Largaespada, David A.…[et al.]. Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-492964
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492964