Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci)
Joint Authors
Guevara-Flores, Alberto
Rendón, Juan L.
Flores-Herrera, Oscar
Pardo, Juan Pablo
del Arenal, Irene P.
Mendoza-Hernández, Guillermo
Source
Journal of Parasitology Research
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Mitochondrial thioredoxin-glutathione reductase was purified from larval Taenia crassiceps (cysticerci).
The preparation showed NADPH-dependent reductase activity with either thioredoxin or GSSG, and was able to perform thiol/disulfide exchange reactions.
At 25∘C specific activities were 437 ± 27 mU mg-1 and 840 ± 49 mU mg-1 with thioredoxin and GSSG, respectively.
Apparent Km values were 0.87 ± 0.04 μM, 41 ± 6 μM and 19 ± 10 μM for thioredoxin, GSSG and NADPH, respectively.
Thioredoxin from eukaryotic sources was accepted as substrate.
The enzyme reduced H2O2 in a NADPH-dependent manner, although with low catalytic efficiency.
In the presence of thioredoxin, mitochondrial TGR showed a thioredoxin peroxidase-like activity.
All disulfide reductase activities were inhibited by auranofin, suggesting mTGR is dependent on selenocysteine.
The reductase activity with GSSG showed a higher dependence on temperature as compared with the DTNB reductase activity.
The variation of the GSSG- and DTNB reductase activities on pH was dependent on the disulfide substrate.
Like the cytosolic isoform, mTGR showed a hysteretic kinetic behavior at moderate or high GSSG concentrations, but it was less sensitive to calcium.
The enzyme was able to protect glutamine synthetase from oxidative inactivation, suggesting that mTGR is competent to contend with oxidative stress.
American Psychological Association (APA)
Guevara-Flores, Alberto& del Arenal, Irene P.& Mendoza-Hernández, Guillermo& Pardo, Juan Pablo& Flores-Herrera, Oscar& Rendón, Juan L.. 2010. Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci). Journal of Parasitology Research،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-493211
Modern Language Association (MLA)
Guevara-Flores, Alberto…[et al.]. Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci). Journal of Parasitology Research No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-493211
American Medical Association (AMA)
Guevara-Flores, Alberto& del Arenal, Irene P.& Mendoza-Hernández, Guillermo& Pardo, Juan Pablo& Flores-Herrera, Oscar& Rendón, Juan L.. Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci). Journal of Parasitology Research. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-493211
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493211