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Purification and Characterization of a Nonenzymatic Neurotoxin from Hippasa partita (Lycosidae) Spider Venom Gland Extract
Joint Authors
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Pharmacy, Health & Medical Sciences
Biology
Medicine
Abstract EN
India is a habitat for nearly one thousand four hundred forty-seven species of spiders under three hundred and sixty-five genera and sixty families.
Our initial survey on toxic bite by spider revealed severe edema, itching, acute pain, and hemorrhage following tissue necrosis, which are the general symptoms of envenomation, but there are no reports of mortality.
Significantly, Hippasa partita spider, commonly called “funnel web spider,” which is endemic in hilly regions of the Western Ghats is responsible for envenomation.
In this study, a nonenzymatic neurotoxin has been purified from H.
partita venom gland extract.
Gel filtration and ion exchange chromatography were used to purify the toxin into homogeneity as shown by SDS-PAGE, RP-HPLC, and MALDI-TOF.
Neurotoxin is devoid of enzymatic activities but causes intense neurotoxic symptoms.
Neurotoxin is found to inhibit the twitch response of sciatic nerve gastrocnemius muscle preparation and is found to be postsynaptic in action.
Neurotoxin is devoid of coagulant activity, edema, and hemorrhage and is nonlethal to mice (up to 5 mg/kg body weight).
In conclusion, a neurotoxin, which is a principle agent in whole venom responsible for induced neurotoxic symptoms, has been purified and characterized.
American Psychological Association (APA)
Nagaraju, S.& Kemparaju, K.. 2013. Purification and Characterization of a Nonenzymatic Neurotoxin from Hippasa partita (Lycosidae) Spider Venom Gland Extract. Journal of Toxins،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-493218
Modern Language Association (MLA)
Nagaraju, S.& Kemparaju, K.. Purification and Characterization of a Nonenzymatic Neurotoxin from Hippasa partita (Lycosidae) Spider Venom Gland Extract. Journal of Toxins No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-493218
American Medical Association (AMA)
Nagaraju, S.& Kemparaju, K.. Purification and Characterization of a Nonenzymatic Neurotoxin from Hippasa partita (Lycosidae) Spider Venom Gland Extract. Journal of Toxins. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-493218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493218