Relative Expression Levels Rather Than Specific Activity Plays the Major Role in Determining In Vivo AKT Isoform Substrate Specificity
Joint Authors
Hannan, Ross D.
Hannan, Katherine M.
Cristiano, Briony E.
House, Colin M.
Pearson, Richard B.
Lee, Rachel S.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-22
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
The AKT protooncogene mediates many cellular processes involved in normal development and disease states such as cancer.
The three structurally similar isoforms: AKT1, AKT2, and AKT3 exhibit both functional redundancy and isoform-specific functions; however the basis for their differential signalling remains unclear.
Here we show that in vitro, purified AKT3 is ∼47-fold more active than AKT1 at phosphorylating peptide and protein substrates.
Despite these marked variations in specific activity between the individual isoforms, a comprehensive analysis of phosphorylation of validated AKT substrates indicated only subtle differences in signalling via individual isoforms in vivo.
Therefore, we hypothesise, at least in this model system, that relative tissue/cellular abundance, rather than specific activity, plays the dominant role in determining AKT substrate specificity in situ.
American Psychological Association (APA)
Lee, Rachel S.& House, Colin M.& Cristiano, Briony E.& Hannan, Ross D.& Pearson, Richard B.& Hannan, Katherine M.. 2011. Relative Expression Levels Rather Than Specific Activity Plays the Major Role in Determining In Vivo AKT Isoform Substrate Specificity. Enzyme Research،Vol. 2011, no. 2011, pp.1-18.
https://search.emarefa.net/detail/BIM-493300
Modern Language Association (MLA)
Lee, Rachel S.…[et al.]. Relative Expression Levels Rather Than Specific Activity Plays the Major Role in Determining In Vivo AKT Isoform Substrate Specificity. Enzyme Research No. 2011 (2011), pp.1-18.
https://search.emarefa.net/detail/BIM-493300
American Medical Association (AMA)
Lee, Rachel S.& House, Colin M.& Cristiano, Briony E.& Hannan, Ross D.& Pearson, Richard B.& Hannan, Katherine M.. Relative Expression Levels Rather Than Specific Activity Plays the Major Role in Determining In Vivo AKT Isoform Substrate Specificity. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-18.
https://search.emarefa.net/detail/BIM-493300
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493300