Characterization of Plasmid pPO1 from the Hyperacidophile Picrophilus oshimae
Joint Authors
Voss, Jörn
Angelov, Angel
Liebl, Wolfgang
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-20
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Picrophilus oshimae and Picrophilus torridus are free-living, moderately thermophilic and acidophilic organisms from the lineage of Euryarchaeota.
With a pH optimum of growth at pH 0.7 and the ability to even withstand molar concentrations of sulphuric acid, these organisms represent the most extreme acidophiles known.
So far, nothing is known about plasmid biology in these hyperacidophiles.
Also, there are no genetic tools available for this genus.
We have mobilized the 7.6 Kbp plasmid from P.
oshimae in E.
coli by introducing origin-containing transposons and described the plasmid in terms of its nucleotide sequence, copy number in the native host, mode of replication, and transcriptional start sites of the encoded ORFs.
Plasmid pPO1 may encode a restriction/modification system in addition to its replication functions.
The information gained from the pPO1 plasmid may prove useful in developing a cloning system for this group of extreme acidophiles.
American Psychological Association (APA)
Angelov, Angel& Voss, Jörn& Liebl, Wolfgang. 2011. Characterization of Plasmid pPO1 from the Hyperacidophile Picrophilus oshimae. Archaea،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-493457
Modern Language Association (MLA)
Angelov, Angel…[et al.]. Characterization of Plasmid pPO1 from the Hyperacidophile Picrophilus oshimae. Archaea No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-493457
American Medical Association (AMA)
Angelov, Angel& Voss, Jörn& Liebl, Wolfgang. Characterization of Plasmid pPO1 from the Hyperacidophile Picrophilus oshimae. Archaea. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-493457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493457