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Feasibility of Whole RNA Sequencing from Single-Cell mRNA Amplification
Joint Authors
Hu, Hongliang
Xu, Yunbo
Zheng, Jie
Li, Biaoru
Source
Genetics Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Single-cell sampling with RNA-seq analysis plays an important role in reference laboratory; cytogenomic diagnosis for specimens on glass-slides or rare cells in circulating blood for tumor and genetic diseases; measurement of sensitivity and specificity in tumor-tissue genomic analysis with mixed-cells; mechanism analysis of differentiation and proliferation of cancer stem cell for academic purpose.
Our single- cell RNA-seq technique shows that fragments were 250–450 bp after fragmentation, amplification, and adapter addition.
There were 11.6 million reads mapped in raw sequencing reads (19.6 million).
The numbers of mapped genes, mapped transcripts, and mapped exons were 31,332, 41,210, and 85,786, respectively.
All QC results demonstrated that RNA-seq techniques could be used for single-cell genomic performance.
Analysis of the mapped genes showed that the number of genes mapped by RNA-seq (6767 genes) was much higher than that of differential display (288 libraries) among similar specimens which we have developed and published.
The single-cell RNA-seq can detect gene splicing using different subtype TGF-beta analysis.
The results from using Q-rtPCR tests demonstrated that sensitivity is 76% and specificity is 55% from single-cell RNA-seq technique with some gene expression missing (2/8 genes).
However, it will be feasible to use RNA-seq techniques to contribute to genomic medicine at single-cell level.
American Psychological Association (APA)
Xu, Yunbo& Hu, Hongliang& Zheng, Jie& Li, Biaoru. 2013. Feasibility of Whole RNA Sequencing from Single-Cell mRNA Amplification. Genetics Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-493511
Modern Language Association (MLA)
Xu, Yunbo…[et al.]. Feasibility of Whole RNA Sequencing from Single-Cell mRNA Amplification. Genetics Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-493511
American Medical Association (AMA)
Xu, Yunbo& Hu, Hongliang& Zheng, Jie& Li, Biaoru. Feasibility of Whole RNA Sequencing from Single-Cell mRNA Amplification. Genetics Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-493511
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493511