Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin
Joint Authors
Pawlas, Krystyna
Sokołowska, Magdalena
Bal, Wojciech
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Visible-range circular dichroism titrations were used to study Cu(II) binding properties of Multimetal Binding Site (MBS) of Human Serum Albumin (HSA).
The formation of ternary MBS-Cu(II)-Buffer complexes at pH 7.4 was positively verified for sodium phosphate, Tris, and Hepes, the three most common biochemical buffers.
The phosphate > Hepes > Tris order of affinities, together with strong spectral changes induced specifically by Tris, indicates the presence of both Buffer-Cu(II) and Buffer-HSA interactions.
All complexes are strong enough to yield a nearly 100% ternary complex formation in 0.5 mM HSA dissolved in 100 mM solutions of respective buffers.
The effects of warfarin and ibuprofen, specific ligands of hydrophobic pockets I and II in HSA on the Cu(II) binding to MBS were also investigated.
The effects of ibuprofen were negligible, but warfarin diminished the MBS affinity for Cu(II) by a factor of 20, as a result of indirect conformational effects.
These results indicate that metal binding properties of MBS can be modulated directly and indirectly by small molecules.
American Psychological Association (APA)
Sokołowska, Magdalena& Pawlas, Krystyna& Bal, Wojciech. 2010. Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin. Bioinorganic Chemistry and Applications،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-493610
Modern Language Association (MLA)
Sokołowska, Magdalena…[et al.]. Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin. Bioinorganic Chemistry and Applications No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-493610
American Medical Association (AMA)
Sokołowska, Magdalena& Pawlas, Krystyna& Bal, Wojciech. Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin. Bioinorganic Chemistry and Applications. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-493610
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493610