Development and Validation of Stability Indicating HPTLC and HPLC Methods for Simultaneous Determination of Telmisartan and Atorvastatin in Their Formulations
Joint Authors
Ilango, Kaliappan
Shiji Kumar, Pushpangadhan S.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The present study describes development and subsequent validation of stability indicating HPLC and HPTLC methods for simultaneous estimation of Telmisartan (TLM) and Atorvastatin (ATV) in their combined formulation.
The proposed RP-HPLC method utilizes a Phenomenex Luna C18 column using acetonitrile: 0.025 M ammonium acetate (38 : 52%, v/v) as mobile phase (pH 3.8), flow rate of 1.0 mL/min.
Quantification was achieved with UV detection at 281 nm over concentration range of 12 to 72 μg/mL for TLM and 3 to 18 μg/mL for ATV respectively.
In HPTLC, separations were performed on silica gel 60 F254 using toluene-methanol-ethyl acetate-acetic acid (5 : 1 : 1 : 0.3, v/v) as mobile phase.
The compact bands of TLM and ATV at Rf 0.37 ± 0.02 and 0.63 ± 0.01 respectively were scanned at 279 nm.
Linear regression analysis revealed linearity in the range of 40 to 240 ng/band for TLM and 10 to 60 ng/band for ATV respectively.
For both the methods, dosage form was exposed to thermal, photolytic, acid, alkali and oxidative stress.
The methods distinctly separated the drugs and degradation products even in actual samples.
In conclusion, the proposed HPLC and HPTLC methods were appropriate for routine quantification of TLM and ATV in tablet formulation.
American Psychological Association (APA)
Ilango, Kaliappan& Shiji Kumar, Pushpangadhan S.. 2013. Development and Validation of Stability Indicating HPTLC and HPLC Methods for Simultaneous Determination of Telmisartan and Atorvastatin in Their Formulations. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-493646
Modern Language Association (MLA)
Ilango, Kaliappan& Shiji Kumar, Pushpangadhan S.. Development and Validation of Stability Indicating HPTLC and HPLC Methods for Simultaneous Determination of Telmisartan and Atorvastatin in Their Formulations. Journal of Chemistry No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-493646
American Medical Association (AMA)
Ilango, Kaliappan& Shiji Kumar, Pushpangadhan S.. Development and Validation of Stability Indicating HPTLC and HPLC Methods for Simultaneous Determination of Telmisartan and Atorvastatin in Their Formulations. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-493646
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493646