Iodothyronine Interactions with the System L1 Amino Acid Exchanger in 3T3-L1 Adipocytes
Joint Authors
Mitchell, Fiona E.
Roy, Lisa A.
Taylor, Peter M.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Thyroid hormones enter isolated white adipocytes largely by a System L1-type amino acid transporter en route to exerting genomic actions.
Differentiated 3T3-L1 mouse adipocytes in culture express mRNA for LAT1 (the catalytic subunit of high-affinity System L1).
L-[125I]-T3 uptake into 3T3-L1 adipocytes included a substantial saturable component inhibited by leucine.
L-[3H]phenylalanine uptake into 3T3-L1 cells was saturable (Km of 31 μM), competitively inhibited by T3 (Ki of 1.2 μM) and blocked by leucine, BCH, and rT3 as expected for substrate interactions of System L1.
Efflux of preloaded L-[3H]phenylalanine from 3T3-L1 adipocytes was trans stimulated by external leucine, demonstrating the obligatory exchange mechanism of System L1 transport.
T3 (10 μM) did not significantly trans stimulate L-[3H]phenylalanine efflux, but did competitively inhibit the trans stimulatory effect of 10 μM leucine.
The results highlight strong competitive interactions between iodothyronines (T3, rT3) and amino acids for transport by System L1 in adipocytes, which may impact cellular iodothyronine exchanges during altered states of protein nutrition.
American Psychological Association (APA)
Mitchell, Fiona E.& Roy, Lisa A.& Taylor, Peter M.. 2010. Iodothyronine Interactions with the System L1 Amino Acid Exchanger in 3T3-L1 Adipocytes. Journal of Thyroid Research،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-493725
Modern Language Association (MLA)
Mitchell, Fiona E.…[et al.]. Iodothyronine Interactions with the System L1 Amino Acid Exchanger in 3T3-L1 Adipocytes. Journal of Thyroid Research No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-493725
American Medical Association (AMA)
Mitchell, Fiona E.& Roy, Lisa A.& Taylor, Peter M.. Iodothyronine Interactions with the System L1 Amino Acid Exchanger in 3T3-L1 Adipocytes. Journal of Thyroid Research. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-493725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493725