Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods
Joint Authors
Stefan-van Staden, Raluca-Ioana
Nejem, R'afat Mahmoud
Issa, Mahmoud Mohamed
Abu Shanab, Alaa
Source
International Journal of Spectroscopy
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of ternary mixtures without any chemical pretreatment.
The first method is based on modification of H-point standard addition method which permits simultaneous analysis of three species from a unique calibration set by making the simultaneous addition of the three analytes.
Quotient between the spectra of aspirin, atorvastatin, and clopidogrel was obtained and the results showed that simultaneous determination of aspirin, atorvastatin, and clopidogrel can be obeyed in the linear range 2.5–20 μg mL−1 of aspirin, 2.5–17.5 μg mL−1 of atorvastatin, and 2.5–20 μg mL−1 of clopidogrel in ternary mixture.
The second method is based on the combination of the first derivative spectra and Cramer's matrix rule.
In the matrix calculation, clopidogrel has zero crossing point at 316.8 and 212 nm, while for atorvastatin the zero crossing point at 250 nm where the matrix is greatly simplified and easily solved.
The linear concentration ranges were 2.5–20 μg mL−1 aspirin, 2.5–17.5 μg mL−1 atorvastatin and 2.5–20 μg mL−1 clopidogrel in ternary mixtures.
The results proved that the simultaneous determination of aspirin, atorvastatin, and clopidogrel could be obeyed.
Both methods were applied for capsules containing the three ingredients and results were in good concordance with alternative liquid chromatography.
American Psychological Association (APA)
Issa, Mahmoud Mohamed& Nejem, R'afat Mahmoud& Abu Shanab, Alaa& Stefan-van Staden, Raluca-Ioana. 2013. Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods. International Journal of Spectroscopy،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-493784
Modern Language Association (MLA)
Issa, Mahmoud Mohamed…[et al.]. Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods. International Journal of Spectroscopy No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-493784
American Medical Association (AMA)
Issa, Mahmoud Mohamed& Nejem, R'afat Mahmoud& Abu Shanab, Alaa& Stefan-van Staden, Raluca-Ioana. Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods. International Journal of Spectroscopy. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-493784
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493784