Comparative Proteomic Analysis of Proteins Involved in the Tumorigenic Process of Seminal Vesicle Carcinoma in Transgenic Mice
Joint Authors
Hsu, Wen-Lian
Chen, Chein-Hung
Chou, Chuan-Kai
Chang, Wei-Chao
Zhuo, Yu-Xing
Chen, Chung-Hsuan
Li, Sheng-Hsiang
Tsou, Chih-Chiang
Source
International Journal of Proteomics
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-12
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
We studied the seminal vesicle secretion (SVS) of transgenic mice by using one-dimensional gel electrophoresis combined with LTQ-FT ICR MS analysis to explore protein expression profiles.
Using unique peptide numbers as a cut-off criterion, 79 proteins were identified with high confidence in the SVS proteome.
Label-free quantitative analysis was performed by using the IDEAL_Q software program.
Furthermore, western blot assays were performed to validate the expression of seminal vesicle proteins.
Sulfhydryl oxidase 1, glia-derived nexin, SVS1, SVS3, and SVS6 showed overexpression in SVS during cancer development.
With high sequence similarity to human semenogelin, SVS2 is the most abundance protein in SVS and is dramatically decreased during the tumorigenic process.
Our results indicate that these protein candidates could serve as potential targets for monitoring seminal vesicle carcinoma.
Moreover, this information can provide clues for investigating seminal vesicle secretion-containing seminal plasma for related human diseases.
American Psychological Association (APA)
Chang, Wei-Chao& Chou, Chuan-Kai& Tsou, Chih-Chiang& Li, Sheng-Hsiang& Chen, Chein-Hung& Zhuo, Yu-Xing…[et al.]. 2010. Comparative Proteomic Analysis of Proteins Involved in the Tumorigenic Process of Seminal Vesicle Carcinoma in Transgenic Mice. International Journal of Proteomics،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-493804
Modern Language Association (MLA)
Chang, Wei-Chao…[et al.]. Comparative Proteomic Analysis of Proteins Involved in the Tumorigenic Process of Seminal Vesicle Carcinoma in Transgenic Mice. International Journal of Proteomics No. 2010 (2010), pp.1-14.
https://search.emarefa.net/detail/BIM-493804
American Medical Association (AMA)
Chang, Wei-Chao& Chou, Chuan-Kai& Tsou, Chih-Chiang& Li, Sheng-Hsiang& Chen, Chein-Hung& Zhuo, Yu-Xing…[et al.]. Comparative Proteomic Analysis of Proteins Involved in the Tumorigenic Process of Seminal Vesicle Carcinoma in Transgenic Mice. International Journal of Proteomics. 2010. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-493804
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493804