In Vitro Anti-Candida Activity of Lidocaine and Nitroglycerin : Alone and Combined

Abstract EN

The aim of this work was to study the anti-Candida activity of lidocaine and nitroglycerin alone and in combination.

Ten Candida strains were included, corresponding to 1 collection type strain (ATCC 10231) and 9 clinical isolates: 4 C.

albicans, 2 C.

glabrata, 1 C.

tropicalis, 1 C.

krusei, and 1 C.

parapsilosis.

The CLSI reference M27-A3 micromethod was used to determine the anti-Candida activity of the drugs alone; minimal inhibitory and lethal concentrations were determined.

The classic checkboard technique was used to determine the activity of combined drugs.

Lidocaine fungicidal effect was dosedependent.

Nitroglycerin exhibited a higher effect.

The drugs combination resulted in a reduction of the inhibitory concentration, corresponding to an additive effect.

In conclusion, both drugs exhibited an interesting anti-Candida activity.

The combination of lidocaine with nitroglycerin was shown to have an additive effect against Candida spp., predicting the interest to include, in the future, these drugs in a new delivery system for the treatment of mucocutaneous candidosis.