A novel aspartic protease with HIV-1 reverse transcriptase inhibitory activity from fresh fruiting bodies of the wild mushroom Xylaria hypoxylon
Joint Authors
Zhang, Rui-Ying
Zhang, Guo-Qing
Hu, Dan-Dan
Ng, Tzi Bun
Wang, He-Xiang
Hu, Qing-Xiu
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A novel aspartic protease with HIV-1 RT inhibitory activity was isolated and characterized from fruiting bodies of the wild mushroom Xylaria hypoxylon.
The purification protocol comprised distilled water homogenization and extraction step, three ion exchange chromatographic steps (on DEAE-cellulose, Q-Sepharose, and CM-cellulose in succession), and final purification was by FPLC on Superdex 75.
The protease was adsorbed on all the three ion exchangers.
It was a monomeric protein with a molecular mass of 43 kDa as estimated by SDS-PAGE and FPLC.
Its N-terminal amino acid sequence was HYTELLSQVV, which exhibited no sequence homology to other proteases reported.
The activity of the protease was adversely affected by Pepstatin A, indicating that it is an aspartic protease.
The protease activity was maximal or nearly so in the pH range 6–8 and in the temperature range 35–60°C.
The purified enzyme exhibited HIV-1 RT inhibitory activity with an IC50 value of 8.3 μM, but was devoid of antifungal, ribonuclease, and hemagglutinating activities.
American Psychological Association (APA)
Hu, Qing-Xiu& Zhang, Guo-Qing& Zhang, Rui-Ying& Hu, Dan-Dan& Wang, He-Xiang& Ng, Tzi Bun. 2012. A novel aspartic protease with HIV-1 reverse transcriptase inhibitory activity from fresh fruiting bodies of the wild mushroom Xylaria hypoxylon. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-493972
Modern Language Association (MLA)
Hu, Qing-Xiu…[et al.]. A novel aspartic protease with HIV-1 reverse transcriptase inhibitory activity from fresh fruiting bodies of the wild mushroom Xylaria hypoxylon. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-493972
American Medical Association (AMA)
Hu, Qing-Xiu& Zhang, Guo-Qing& Zhang, Rui-Ying& Hu, Dan-Dan& Wang, He-Xiang& Ng, Tzi Bun. A novel aspartic protease with HIV-1 reverse transcriptase inhibitory activity from fresh fruiting bodies of the wild mushroom Xylaria hypoxylon. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-493972
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6-8
Record ID
BIM-493972