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Antiviral Protein of Momordica charantia L. Inhibits Different Subtypes of Influenza A
Joint Authors
Pongthanapisith, Viroj
Puthavathana, Pilaipan
Ikuta, Kazuyoshi
Leelamanit, Wichet
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The new antiviral activity of the protein extracted from Momordica charantia was determined with different subtypes of influenza A.
The protein was purified from the seed of M.
charantia using an anion exchanger and a Fast Protein Liquid Chromatography (FPLC) system.
At the concentration of 1.401 mg/mL, the protein did not exhibit cytotoxicity in Madin-Darby canine kidney cells (MDCK) but inhibited 1×105 FFU influenza A/PR/8/34 H1N1 virus at 56.50%, 65.72%, and 100% inhibition by the protein treated before the virus (pretreated), the protein treated alongside with the virus (simultaneously treated), and the protein treated after the virus (posttreated) during incubation, respectively.
Using 5, 25, and 100 TCID50 of influenza A/New Caledonia/20/99 H1N1, A/Fujian/411/01 H3N2 and A/Thailand/1(KAN-1)/2004 H5N1, the IC50 was calculated to be 100, 150, and 200; 75, 175, and 300; and 40, 75, and 200 μg/mL, respectively.
Our present finding indicated that the plant protein inhibited not only H1N1 and H3N2 but also H5N1 subtype.
As a result of the broad spectrum of its antiviral activity, this edible plant can be developed as an effective therapeutic agent against various and even new emerging subtypes of influenza A.
American Psychological Association (APA)
Pongthanapisith, Viroj& Ikuta, Kazuyoshi& Puthavathana, Pilaipan& Leelamanit, Wichet. 2013. Antiviral Protein of Momordica charantia L. Inhibits Different Subtypes of Influenza A. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-493984
Modern Language Association (MLA)
Pongthanapisith, Viroj…[et al.]. Antiviral Protein of Momordica charantia L. Inhibits Different Subtypes of Influenza A. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-493984
American Medical Association (AMA)
Pongthanapisith, Viroj& Ikuta, Kazuyoshi& Puthavathana, Pilaipan& Leelamanit, Wichet. Antiviral Protein of Momordica charantia L. Inhibits Different Subtypes of Influenza A. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-493984
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493984