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Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung
Joint Authors
Li, Mingcai
Zhou, Yanchun
Chen, Sui
Wu, Yan
Liu, Xiaojin
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Interleukin (IL)-33 is the latest member of IL-1 cytokine family.
In this study, the cloning, expression, purification, and polyclonal antibody preparation of mouse IL-33 were described.
The coding region of IL-33 mature protein was cloned into the prokaryotic expression vector pET-44.
The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia coli.
The expressed soluble protein was purified by immobilized metal-ion affinity chromatography using Ni2+-nitrilotriacetic acid agarose.
The rabbits were immunized with the purified recombinant protein.
The obtained antiserum was precipitated by saturated ammonium sulfate and then purified by Protein A affinity chromatography.
The sensitivity and specificity of the antibodies were evaluated by enzyme-linked immunosorbent assay and immunohistochemistry.
The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein.
Significant expression of IL-33 was seen in mouse liver and lung tissues determined with the anti-IL-33.
The production of the polyclonal antibody against IL-33 provides a good tool for studying the biofunctions of IL-33.
American Psychological Association (APA)
Liu, Xiaojin& Wu, Yan& Li, Mingcai& Chen, Sui& Zhou, Yanchun. 2009. Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-493997
Modern Language Association (MLA)
Liu, Xiaojin…[et al.]. Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-493997
American Medical Association (AMA)
Liu, Xiaojin& Wu, Yan& Li, Mingcai& Chen, Sui& Zhou, Yanchun. Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-493997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493997