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Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus : Analysis of Different Immunization Strategies
Joint Authors
Sun, Xiuqin
Zhang, Jinxing
Wu, Suqi
Zheng, Fengrong
Liu, Hongzhan
Wu, Xing'an
Li, Jiye
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Here, we report the construction of a vaccine against lymphocystis disease virus (LCDV) using nucleic acid vaccination technology.
A fragment of the major capsid protein encoding gene from an LCDV isolated from China (LCDV-cn) was cloned into an eukaryotic expression vector pEGFP-N2, yielding a recombinant plasmid pEGFP-N2-LCDV-cn0.6 kb.
This plasmid was immediately expressed after liposomal transfer into the Japanese flounder embryo cell line.
The recombinant plasmid was inoculated into Japanese flounder via two routes (intramuscular injection and hypodermic injection) at three doses (0.1, 5, and 15 μg), and then T-lymphopoiesis in different tissues and antibodies raised against LCDV were evaluated.
The results indicated that this recombinant plasmid induced unique humoral or cell-mediated immune responses depending on the inoculation route and conferred immune protection.
Furthermore, the humoral immune responses and protective effects were significantly increased at higher vaccine doses via the two injection routes.
Plasmid pEGFP-N2-LCDV0.6 kb is therefore a promising vaccine candidate against LCDV in Japanese flounder.
American Psychological Association (APA)
Zheng, Fengrong& Sun, Xiuqin& Wu, Xing'an& Liu, Hongzhan& Li, Jiye& Wu, Suqi…[et al.]. 2011. Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus : Analysis of Different Immunization Strategies. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-493999
Modern Language Association (MLA)
Zheng, Fengrong…[et al.]. Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus : Analysis of Different Immunization Strategies. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-493999
American Medical Association (AMA)
Zheng, Fengrong& Sun, Xiuqin& Wu, Xing'an& Liu, Hongzhan& Li, Jiye& Wu, Suqi…[et al.]. Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus : Analysis of Different Immunization Strategies. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-493999
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493999