Description of a Novel Adhesin of Mycobacterium avium Subsp. paratuberculosis

Joint Authors

Malchiodi, Emilio
Echeverria-Valencia, Gabriela
Viale, Mariana Noelia
Mon, María Laura
Santangelo, María de la Paz
Gioffre, Andrea Karina
Fernandez, Marisa
Romano, María Isabel
Romasanta, Pablo

Source

BioMed Research International

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-07-22

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Medicine

Abstract EN

The binding and ingestion of Mycobacterium avium subsp.

paratuberculosis (MAP) by host cells are fibronectin (FN) dependent.

In several species of mycobacteria, a specific family of proteins allows the attachment and internalization of these bacteria by epithelial cells through interaction with FN.

Thus, the identification of adhesion molecules is essential to understand the pathogenesis of MAP.

The aim of this study was to identify and characterize FN binding cell wall proteins of MAP.

We searched for conserved adhesins within a large panel of surface immunogenic proteins of MAP and investigated a possible interaction with FN.

For this purpose, a cell wall protein fraction was obtained and resolved by 2D electrophoresis.

The immunoreactive spots were identified by MALDI-TOF MS and a homology search was performed.

We selected elongation factor Tu (EF-Tu) as candidate for further studies.

We demonstrated the FN-binding capability of EF-Tu using a ligand blot assay and also confirmed the interaction with FN in a dose-dependent manner by ELISA.

The dissociation constant of EF-Tu was determined by surface plasmon resonance and displayed values within the μM range.

These data support the hypothesis that this protein could be involved in the interaction of MAP with epithelial cells through FN binding.

American Psychological Association (APA)

Viale, Mariana Noelia& Echeverria-Valencia, Gabriela& Romasanta, Pablo& Mon, María Laura& Fernandez, Marisa& Malchiodi, Emilio…[et al.]. 2014. Description of a Novel Adhesin of Mycobacterium avium Subsp. paratuberculosis. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-494041

Modern Language Association (MLA)

Viale, Mariana Noelia…[et al.]. Description of a Novel Adhesin of Mycobacterium avium Subsp. paratuberculosis. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-494041

American Medical Association (AMA)

Viale, Mariana Noelia& Echeverria-Valencia, Gabriela& Romasanta, Pablo& Mon, María Laura& Fernandez, Marisa& Malchiodi, Emilio…[et al.]. Description of a Novel Adhesin of Mycobacterium avium Subsp. paratuberculosis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-494041

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-494041