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Secondary Structural Preferences of Some Antibacterial Cyclooctapeptides in the Presence of Calcium(II)
Joint Authors
Ngu-Schwemlein, Maria
McNeil, Nykia
Lin, Xiuli
Stevens, Tarshona
Source
International Journal of Medicinal Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The purpose of this study is to understand the interactions of some antibacterial cationic amphipathic cyclooctapeptides with calcium(II) and their secondary structural preferences.
The thermodynamic parameters associated with calcium(II) interactions, between the antibacterial active cyclooctapeptides (COP 1–6) and those that did not exhibit significant activities (COP 7–9), were studied by isothermal titration calorimetry.
Calcium(II) binding in the absence and presence of micellar dodecylphosphocholine (DPC), a membrane mimicking detergent, was conducted by circular dichroism (CD).
Both groups of cyclopeptides showed weak binding affinities for calcium(II) (Kb ca.
10−3 M−1).
However, CD data showed that the antimicrobial peptides COP 1–6 adopted a twisted beta-sheet structure (positive CD absorption band at ca.
203 nm) in the presence of calcium(II) in micellar DPC.
In contrast, COP 7–9, which lacked antibacterial activity, adopted a different conformational structure (negative CD absorption band at ca.
203 nm).
These results indicate that these cyclopeptides could adopt secondary structural preferences in the presence of calcium(II) amidst a hydrophobic environment to elicit their antibacterial activity.
These findings could be useful in facilitating the design of cyclopeptide derivatives that can adopt this beta-sheet-like secondary structure and, thereby, provide a useful molecular template for crafting antibacterial compounds.
American Psychological Association (APA)
Stevens, Tarshona& McNeil, Nykia& Lin, Xiuli& Ngu-Schwemlein, Maria. 2012. Secondary Structural Preferences of Some Antibacterial Cyclooctapeptides in the Presence of Calcium(II). International Journal of Medicinal Chemistry،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-494081
Modern Language Association (MLA)
Stevens, Tarshona…[et al.]. Secondary Structural Preferences of Some Antibacterial Cyclooctapeptides in the Presence of Calcium(II). International Journal of Medicinal Chemistry No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-494081
American Medical Association (AMA)
Stevens, Tarshona& McNeil, Nykia& Lin, Xiuli& Ngu-Schwemlein, Maria. Secondary Structural Preferences of Some Antibacterial Cyclooctapeptides in the Presence of Calcium(II). International Journal of Medicinal Chemistry. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-494081
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494081