A Method to Determine Lysine Acetylation Stoichiometries
Joint Authors
Shukla, Anil K.
Moore, Ronald J.
Ansong, Charles
Hixson, Kim K.
Nakayasu, Ernesto S.
Weitz, Karl K.
Sydor, Michael A.
Monroe, Matthew E.
Smith, Richard D.
Qian, Wei-Jun
Adkins, Joshua N.
Petyuk, Vladislav A.
Wu, Si
Pasa-Tolic, Ljiljiana
Kim, Jong-Seo
Source
International Journal of Proteomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Lysine acetylation is a common protein posttranslational modification that regulates a variety of biological processes.
A major bottleneck to fully understanding the functional aspects of lysine acetylation is the difficulty in measuring the proportion of lysine residues that are acetylated.
Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of protein lysine acetylation.
Using this technique, we determined the modification occupancy for ~750 acetylated peptides from mammalian cell lysates.
Furthermore, the acetylation on N-terminal tail of histone H4 was cross-validated by treating cells with sodium butyrate, a potent deacetylase inhibitor, and comparing changes in stoichiometry levels measured by our method with immunoblotting measurements.
Of note we observe that acetylation stoichiometry is high in nuclear proteins, but very low in mitochondrial and cytosolic proteins.
In summary, our method opens new opportunities to study in detail the relationship of lysine acetylation levels of proteins with their biological functions.
American Psychological Association (APA)
Nakayasu, Ernesto S.& Wu, Si& Sydor, Michael A.& Shukla, Anil K.& Weitz, Karl K.& Moore, Ronald J.…[et al.]. 2014. A Method to Determine Lysine Acetylation Stoichiometries. International Journal of Proteomics،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-494137
Modern Language Association (MLA)
Nakayasu, Ernesto S.…[et al.]. A Method to Determine Lysine Acetylation Stoichiometries. International Journal of Proteomics No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-494137
American Medical Association (AMA)
Nakayasu, Ernesto S.& Wu, Si& Sydor, Michael A.& Shukla, Anil K.& Weitz, Karl K.& Moore, Ronald J.…[et al.]. A Method to Determine Lysine Acetylation Stoichiometries. International Journal of Proteomics. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-494137
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494137