Comparative Study of Corneal Endothelial Cell Damage after Femtosecond Laser Assisted Deep Stromal Dissection
Joint Authors
Li, Dongfang
Liu, Ting
Gao, Hua
Sun, Dapeng
Sui, Wenjie
Zhang, Yangyang
Chen, Zhaoli
Zhang, Jingjing
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
To find a relatively safe designed stromal bed thickness to avoid endothelial damage for lamellar keratoplasty with an Allegretto Wavelight FS200 femtosecond laser.
Methods.
Twelve rabbits were randomly divided into 50 μm and 150 μm groups according to the anticipated residue stromal bed thickness preparation with a femtosecond laser.
Six rabbits without laser cutting were used as a control group.
Central endothelial images were analyzed with in vivo confocal microscopy and scanning electron microscopy.
The apoptosis of endothelium was evaluated with Hoechst 33342 staining and a TUNEL assay.
Results.
The endothelium of the 50 μm group had extensive injuries upon in vivo confocal and scanning electron microscopic observation, and minor injuries were observed in the 150 μm group.
Moreover, more apoptotic cells were observed in the 50 μm group.
Conclusions.
When using a FS200 femtosecond laser assisted anterior lamellar keratoplasty, there was minor endothelium damage with a 150 μm stromal bed, and a more than 150 μm thickness stromal bed design may prevent the damage of corneal endothelium.
American Psychological Association (APA)
Liu, Ting& Zhang, Jingjing& Sun, Dapeng& Sui, Wenjie& Zhang, Yangyang& Li, Dongfang…[et al.]. 2014. Comparative Study of Corneal Endothelial Cell Damage after Femtosecond Laser Assisted Deep Stromal Dissection. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-494225
Modern Language Association (MLA)
Liu, Ting…[et al.]. Comparative Study of Corneal Endothelial Cell Damage after Femtosecond Laser Assisted Deep Stromal Dissection. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-494225
American Medical Association (AMA)
Liu, Ting& Zhang, Jingjing& Sun, Dapeng& Sui, Wenjie& Zhang, Yangyang& Li, Dongfang…[et al.]. Comparative Study of Corneal Endothelial Cell Damage after Femtosecond Laser Assisted Deep Stromal Dissection. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-494225
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494225