A Simple Densitometric Method for the Quantification of Inhibitory Neurotransmitter Gamma-Aminobutyric Acid (GABA) in Rat Brain Tissue
Joint Authors
Ramanathan, Muthiah
Saravana Babu, Chidambaram
Ranju, Vijayan
Kalaivani, Periyathambi
Kesavanarayanan, Krishnamoorthy Selvarajan
Source
Chromatography Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-20
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system which is involved in various physiological and pathological processes.
The present study demonstrates a simple high-performance thin-layer chromatography (HPTLC) method which was developed for the estimation of GABA in rat brain tissue.
The method was validated in terms of precision, recovery, reproducibility, and variability.
Instrumental precision was found to be 0.5891% CV and reproducibility of the method was found to be 0.4141% CV.
Interday and intraday precision of the method was found to be 0.9453% and 1.3236% CV, respectively.
Accuracy of the method was checked by the recovery study, and the average recovery of GABA was found to be 97.98% at 40 ng and 96.15% at 80 ng levels.
The present HPTLC method for GABA estimation was found to be simple, precise, reproducible, sensitive, and accurate.
No doubt, this proposed method will be a useful tool for the estimation of GABA in rat brain tissue.
American Psychological Association (APA)
Saravana Babu, Chidambaram& Kesavanarayanan, Krishnamoorthy Selvarajan& Kalaivani, Periyathambi& Ranju, Vijayan& Ramanathan, Muthiah. 2011. A Simple Densitometric Method for the Quantification of Inhibitory Neurotransmitter Gamma-Aminobutyric Acid (GABA) in Rat Brain Tissue. Chromatography Research International،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-494298
Modern Language Association (MLA)
Saravana Babu, Chidambaram…[et al.]. A Simple Densitometric Method for the Quantification of Inhibitory Neurotransmitter Gamma-Aminobutyric Acid (GABA) in Rat Brain Tissue. Chromatography Research International No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-494298
American Medical Association (AMA)
Saravana Babu, Chidambaram& Kesavanarayanan, Krishnamoorthy Selvarajan& Kalaivani, Periyathambi& Ranju, Vijayan& Ramanathan, Muthiah. A Simple Densitometric Method for the Quantification of Inhibitory Neurotransmitter Gamma-Aminobutyric Acid (GABA) in Rat Brain Tissue. Chromatography Research International. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-494298
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494298
