Baicalein Induces Apoptosis and Autophagy via Endoplasmic Reticulum Stress in Hepatocellular Carcinoma Cells
Joint Authors
Jiang, Chunping
Ding, Yitao
Chen, Weibo
Wang, Zhongxia
Zhang, Guang
Wu, Junhua
Luo, Dongjun
Cao, Yin
Liu, Baorui
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-03
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Hepatocellular carcinoma (HCC) remains a disastrous disease and the treatment for HCC is rather limited.
Separation and identification of active compounds from traditionally used herbs in HCC treatment may shed light on novel therapeutic drugs for HCC.
Methods.
Cell viability and colony forming assay were conducted to determine anti-HCC activity.
Morphology of cells and activity of caspases were analyzed.
Antiapoptotic Bcl-2 family proteins and JNK were also examined.
Levels of unfolded protein response (UPR) markers were determined and intracellular calcium was assayed.
Small interfering RNAs (siRNAs) were used to investigate the role of UPR and autophagy in baicalein-induced cell death.
Results.
Among four studied flavonoids, only baicalein exhibited satisfactory inhibition of viability and colony formation of HCC cells within water-soluble concentration.
Baicalein induced apoptosis via endoplasmic reticulum (ER) stress, possibly by downregulating prosurvival Bcl-2 family, increasing intracellular calcium, and activating JNK.
CHOP was the executor of cell death during baicalein-induced ER stress while eIF2α and IRE1α played protective roles.
Protective autophagy was also triggered by baicalein in HCC cells.
Conclusion.
Baicalein exhibits prominent anti-HCC activity.
This flavonoid induces apoptosis and protective autophagy via ER stress.
Combination of baicalein and autophagy inhibitors may represent a promising therapy against HCC.
American Psychological Association (APA)
Wang, Zhongxia& Jiang, Chunping& Chen, Weibo& Zhang, Guang& Luo, Dongjun& Cao, Yin…[et al.]. 2014. Baicalein Induces Apoptosis and Autophagy via Endoplasmic Reticulum Stress in Hepatocellular Carcinoma Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-494310
Modern Language Association (MLA)
Wang, Zhongxia…[et al.]. Baicalein Induces Apoptosis and Autophagy via Endoplasmic Reticulum Stress in Hepatocellular Carcinoma Cells. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-494310
American Medical Association (AMA)
Wang, Zhongxia& Jiang, Chunping& Chen, Weibo& Zhang, Guang& Luo, Dongjun& Cao, Yin…[et al.]. Baicalein Induces Apoptosis and Autophagy via Endoplasmic Reticulum Stress in Hepatocellular Carcinoma Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-494310
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494310