Electrochemical Capacitance DNA Sensing at Hairpin-Modified Au Electrodes
Joint Authors
Rivera-Gandía, Joel
Cabrera, Carlos R.
Vargas-Barbosa, Nella M.
Ortiz-Quiles, Edwin O.
De La Torre-Meléndez, Yarimar
del Mar Maldonado, Maria
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
An interfacial capacitance measurement electrochemical technique has been used for the sensing of self-assembled DNA hairpin probes (M.
tuberculosis and B.
anthracis) attached to Au electrodes.
The double-layer capacitance (Cdl) was determined with electrochemical perturbations from 0.2 V to 0.5 V versus Ag/AgCl at a Au/M.
tuberculosis DNA hairpin probe at surface coverage Au electrodes.
The capacitance study was done at pH 7, which was necessary to maintain the M.
tuberculosis and B.
anthracis DNA probes closed during the electrochemical perturbation.
Detailed experimental analysis carried out by repetitively switching the electrochemical potential between 0.2 and 0.5 V (versus Ag/AgCl) strongly supports the use of capacitance measurements as a tool to detect the hybridization of DNA targets.
A large change in the capacitance deference between 0.2 and 0.5 V was observed in the DNA hybridization process.
Therefore, no fluorophores or secondary transducers were necessary to sense a DNA target for both DNA hairpins.
American Psychological Association (APA)
Rivera-Gandía, Joel& del Mar Maldonado, Maria& De La Torre-Meléndez, Yarimar& Ortiz-Quiles, Edwin O.& Vargas-Barbosa, Nella M.& Cabrera, Carlos R.. 2011. Electrochemical Capacitance DNA Sensing at Hairpin-Modified Au Electrodes. Journal of Sensors،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-494475
Modern Language Association (MLA)
Rivera-Gandía, Joel…[et al.]. Electrochemical Capacitance DNA Sensing at Hairpin-Modified Au Electrodes. Journal of Sensors No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-494475
American Medical Association (AMA)
Rivera-Gandía, Joel& del Mar Maldonado, Maria& De La Torre-Meléndez, Yarimar& Ortiz-Quiles, Edwin O.& Vargas-Barbosa, Nella M.& Cabrera, Carlos R.. Electrochemical Capacitance DNA Sensing at Hairpin-Modified Au Electrodes. Journal of Sensors. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-494475
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494475