Zinc Induced Enzymatic Defense Mechanisms in Rhizoctonia Root Rot Infected Clusterbean Seedlings

Abstract EN

This investigation was planned to determine the effect of different concentrations of zinc (Zn) on biochemical constituents of clusterbean, which play an important role in disease resistance mechanisms.

Clusterbean seedlings were grown with 0, 10, or 20 mg Zn kg−1 soil treatments in earthen pots filled with 700 g inoculated soil.

Soil was inoculated by pretreatment with 250 mg (wet weight) of Rhizoctonia inoculums per pot.

A similar set was maintained in uninoculated soil.

Root rot incidence decreased to 41 and 27 per cent with 10 and 20 mg Zn kg−1 soil treatments, respectively, as compared to 68 percent at control.

Antioxidative enzyme activity (polyphenol oxidase, peroxidase, phenylalanine ammonia lyase, and tyrosine ammonia lyase) increased in inoculated seedlings and was increased further by 20 mg Zn kg−1 soil treatment.

Antioxidative enzymes play an important role against fungal invasion, as peroxidase is involved in the formation of barrier via lignifications at the site of pathogen penetration.

PAL and TAL play a key role in phenylpropanoid metabolism and could perform defense-related functions.

Zn acts as a cofactor for these enzymes, so it can be concluded that Zn may be used as a soil-nutritive agent to increase resistance in plants against fungal diseases.