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Structural Basis for Species Selectivity in the HIV-1 gp120-CD4 Interaction : Restoring Affinity to gp120 in Murine CD4 Mimetic Peptides
Joint Authors
Kassler, Kristin
Meier, Julia
Sticht, Heinrich
Eichler, Jutta
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The first step of HIV-1 infection involves interaction between the viral glycoprotein gp120 and the human cellular receptor CD4.
Inhibition of the gp120-CD4 interaction represents an attractive strategy to block HIV-1 infection.
In an attempt to explore the known lack of affinity of murine CD4 to gp120, we have investigated peptides presenting the putative gp120-binding site of murine CD4 (mCD4).
Molecular modeling indicates that mCD4 protein cannot bind gp120 due to steric clashes, while the larger conformational flexibility of mCD4 peptides allows an interaction.
This finding is confirmed by experimental binding assays, which also evidenced specificity of the peptide-gp120 interaction.
Molecular dynamics simulations indicate that the mCD4-peptide stably interacts with gp120 via an intermolecular β-sheet, while an important salt-bridge formed by a C-terminal lysine is lost.
Fixation of the C-terminus by introducing a disulfide bridge between the N- and C-termini of the peptide significantly enhanced the affinity to gp120.
American Psychological Association (APA)
Kassler, Kristin& Meier, Julia& Eichler, Jutta& Sticht, Heinrich. 2012. Structural Basis for Species Selectivity in the HIV-1 gp120-CD4 Interaction : Restoring Affinity to gp120 in Murine CD4 Mimetic Peptides. Advances in Bioinformatics،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-494621
Modern Language Association (MLA)
Kassler, Kristin…[et al.]. Structural Basis for Species Selectivity in the HIV-1 gp120-CD4 Interaction : Restoring Affinity to gp120 in Murine CD4 Mimetic Peptides. Advances in Bioinformatics No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-494621
American Medical Association (AMA)
Kassler, Kristin& Meier, Julia& Eichler, Jutta& Sticht, Heinrich. Structural Basis for Species Selectivity in the HIV-1 gp120-CD4 Interaction : Restoring Affinity to gp120 in Murine CD4 Mimetic Peptides. Advances in Bioinformatics. 2012. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-494621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494621